IONTOSORB - Bead Cellulose Derivatives
[ Home ] [ Up ] [ Support for Synthesis ] [ Dry Tincture ] [ Aluminium Complexation ] [ Reference Materials ] [ Reference Materials 2 ] [ Aluminium Determination ] [ Metal Oxoanions ] [ Isolation of Lysozyme ]

Magnetic Cation Exchange Isolation of Lysozyme from Native Hen Egg White

Food Technol. Biotechnol., 45 (2007) 355 – 359

Ivo Safarik1,2, Zdenka Sabatkova1, Oldrich Tokar3 and Mirka Safarikova1 

  1. Department of Biomagnetic Techniques, Institute of Systems Biology and Ecology, Academy of Sciences, Na Sadkach 7, CZ-370 05 Ceske Budejovice, Czech Republic

  2. Department of General Biology, Faculty of Biological Sciences, University of South Bohemia, Branisovska 31, CZ-370 05 Ceske Budejovice, Czech Republic

  3. Iontosorb, Anezky Ceske 657/33, CZ-400 07 Usti nad Labem, Czech Republic

 

Summary

Two magnetic macroporous cellulose cation exchangers (Iontosorb MG CM 100 and Iontosorb MG SHP 100) were used for one-step isolation of lysozyme from native, undiluted hen egg white. Highly purified lysozyme (purity >96 %) with specific activity similar to that of commercial lysozyme preparations was obtained in both cases. Carboxymethyl-based cation exchanger exhibited substantially higher capacity for lysozyme; maximum adsorption capacity was 138 mg/mL. The lysozyme-depleted egg white can be used in the same way as the routinely used egg white because no dilution of this material was necessary during the purification process.

This SEPARATE (article).