Iontosorb BLUE 2
is an adsorbent based on bead-form macroporous cellulose which is used for affinity chromatography (Dye Ligand Chromatography). It contains the covalently bound dye Reactive Blue 2:
Iontosorb BLUE 2 is a specific sorbent with affinity to wide group of enzymes. Iontosorb BLUE 2 binds also the serum albumin and can be used for the selective removal of albumin from other plasma proteins.
Applications of Iontosorb BLUE 2
Iontosorb BLUE 2 can be used in the following applications:
COMPETITIVE ELUTION OF LACTATE DEHYDROGENASE FROM CIBACRON BLUE CELLULOSE (Iontosorb BLUE-2) WITH CIBACRON BLUE-DEXTRAN
D. Mislovicova, P. Gemeiner, E. Stratilova and M. Horvathova
Journal of Chromatography, 510 (1990) 197-204
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Elution
profiles of LDH of beef muscle extract from Iontosorb BLUE-2
Eluting agents: (squares) 3 M KCl (full circle) 1mM NADH (circle) 50 mM Cibacron Blue Dextran T 10 (full squares) equilibration 20 mM phosphate buffer (pH 8.5) |
Original articles as full texts are available in the information CD of the firm Iontosorb.
Technical Parameters
| Porozity Type | Particle Size [ um ] |
| BLUE-2 100 | 30 - 50 |
| 50 - 80 | |
| 80 - 100 | |
| 100 - 250 | |
| 250 - 500 | |
| BLUE-2 200 | 50 - 80 |
| 80 - 100 | |
| 100 - 250 | |
| 250 - 500 | |
| BLUE-2 500 | 50 - 80 |
| 80 - 100 | |
| 100 - 250 | |
| 250 - 500 |