IONTOSORB - Bead Cellulose Derivatives
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Iontosorb BLUE 2

is an adsorbent based on bead-form macroporous cellulose which is used for affinity chromatography (Dye Ligand Chromatography). It contains the covalently bound dye Reactive Blue 2:

Chemical Structure (3281 bytes)

Iontosorb BLUE 2 is a specific sorbent with affinity to wide group of enzymes. Iontosorb BLUE 2 binds also the serum albumin and can be used for the selective removal of albumin from other plasma proteins.

Applications of Iontosorb BLUE 2

COMPETITIVE ELUTION OF LACTATE DEHYDROGENASE FROM CIBACRON BLUE CELLULOSE (Iontosorb BLUE-2) WITH CIBACRON BLUE-DEXTRAN

D. Mislovicova, P. Gemeiner, E. Stratilova and M. Horvathova

Journal of Chromatography, 510 (1990) 197-204

Elution profiles of LDH of beef muscle extract from Iontosorb BLUE-2

Eluting agents: (squares) 3 M KCl

(full circle) 1mM NADH

(circle) 50 mM Cibacron Blue Dextran T 10

(full squares) equilibration 20 mM phosphate buffer (pH 8.5)

Technical Parameters

Porozity Type	Particle Size [ um ]
BLUE-2 100	30 - 50
	50 - 80
	80 - 100
	100 - 250
	250 - 500
BLUE-2 200	50 - 80
	80 - 100
	100 - 250
	250 - 500
BLUE-2 500	50 - 80
	80 - 100
	100 - 250
	250 - 500