IONTOSORB - Bead Cellulose Derivatives
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Iontosorb SHP

is a strongly acidic cation exchanger based on Bead Cellulose with 3-sulpho-2-hydroxypropyl- groups:

Its total volume capacity can move in the range 0.04 to 0.15 mmol/ml, adequate mass capacity is 0.15 to 0.45 mmol/g dry matter. According to customer´s demand the sorbents volume capacity can be varied in the range given by the mass capacity and water content. Water content moves in connection with   the volume capacity in the range 58 to 85 %.

Iontosorb SHP can be used in the chromatographic separations of proteins, peptides, amino acids, nucleic acid components and other biological substances.

Applications of Iontosorb SHP

Iontosorb SHP can be used in the following applications:

• Ion Exchange Chromatography
• Affinity Chromatography
• Isolation of  LYSOZYME

MAGNETIC CATION EXCHANGE ISOLATION OF LYSOZYME FROM NATIVE HEG EGG WHITE

I. Safarik, Z. Sabatkova, O. Tokar, M. Safarikova

Food Technol. Biotechnol., 45 (2007) 355-359

Fast protein liquid chromatography of original egg white (top), eluate from Iontosorb MG CM 100 (middle) and eluate from Iontosorb MG SHP 100 (bottom)

Set of five overlapping spectra of a lysozyme peak (retention time8.424 min) between 190 and 400 nm obtained ofter Iontosorb MG SHP isolation The spectra were taken 8.068, 8.255, 8.415, 8.575 and 8.735 min, respectively

Equilibrium adsorption isotherms of hen egg white lysozyme using Iontosorb MG CM 100 (full triangles) and Iontosorb MG SHP 100 (full squares) as adsorbents Ceq - equilibrium liquid-phase concentration of the unadsorbed (free) lysozyme; qeq - equilibrium solid-phase concentration of the adsorbed lysozyme

Original articles as full texts are available in the information CD of the firm Iontosorb or Isolation of  LYSOZYME .

Technical Parameters