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Applications:
HYDROPHOBIC INTERACTION CHROMATOGRAPHY
- PURIFICATION OF CARP (Cyprinus carpio) HEPATOPANCREATIC LACTATE DEHYDROGENASE
(EC 1.1.1.27) (Iontosorb PHENYL)
- Kminkova M., Kucera J.: Prep. Biochem.
& Biotechnol., 28(4) (1998)
313-317
Lactate dehydrogenase (LDH, EC 1.1.1.27) was isolated in 86 % purity from carp
(Cyprinus carpio) hepatopancreas using agarose T-gel (mercaptoethanol bound to agarose via
divinylsulphone) affinity chromatography medium and Iontosorb PHENYL hydrophobic
chromatography medium. Gel filtration chromatography of the LDH obtained shows molecular
weight to be 26,900 Da.
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