IONTOSORB« - Bead Cellulose
Macroporous Bead Cellulose - General Information
Macroporous Bead Cellulose MT - Gel Filtration Media for Biomolecule Separations
Macroporous bead cellulose has a hydrophilic matrix and spherical particles. It is produced in several porosity types, marked MT 50, MT 100, MT 200 and MT 500, and various particle size. Macroporous Bead Cellulose, MT is a highly porous regenerated cellulose with heterogenous matrix (partially microcrystalline) and with polymer structure stabilized by hydrogen bonds only - there are no covalent cross-linkings.
One of working sites, which use to deal with a research of bead cellulose applications, was: Department of Glycobiotechnology, Institute of Chemistry, Slovak Academy of Science, Bratislava, Slovakia.
Flow characteristics of Macroporous Bead Cellulose MT types with various particle sizes
(1) For distilled water at 20 oC.
Optimum*) pressure in column during packing with Macroporous Bead Cellulose MT
*) pressure at which maximum of efficiently is reached
Maximum*) flow and pressure throughput for Macroporous Bead Cellulose MT
*) maximum of flow rate or pressure at which column is stabill not
Total Pore Volume for Various Porozities of Macroporous Bead Cellulose MT
The Pore Volume Determined as W.R.
Average values of Total Pore Volume for various porozities of Macroporous Bead Cellulose MT
By their pressure stability Macroporous Bead Cellulose MT belongs to the category of swollen semi-rigid gels for low-pressure chromatography. The gels are characterized by excellent mechanical properties.
Flow characteristics of Macroporous Bead Cellulose MT types Gel bed 1 x 60 cm, particle size 50 - 100 Ám for all types
Column: 10 x 660 mm, Eluent: destilled water, Packing Flow Rate: 760 cm/hr
Column: 10 x 660 mm, Eluent: destilled water, Packing Pressure: 2.5 bar
Column: 10 x 660 mm, Eluent: destilled water, Packing Pressure: 1.75 bar
Column: 10 x 660 mm, Eluent: destilled water, Packing Pressure: 1.25 bar
Column: 10 x 660 mm, Eluent: buffer phosphate 0.2 M pH = 7, Packing Pressure: 1 bar
Agarose = agarose gel, Japan = Japan Cellulose 2000
Column: 10 x 660 mm, Eluent: destilled water
Japan = Japan Cellulose 90, 300, 1000, Dextran = dextran gel 100
not include the interstitial spaces
The resutls in this table were measured by:
Ing. Karel Jerabek, Ph.D.
Institute of Chemical Process Fundamentals
165 02 Prague 6
Size Exclusion Limits for Properties (Porosity) of Individual MT types
Macroporous Bead Cellulose MT types are highly porous gels well accessible for large macromolecules, as it is shown in this Figures.
Selectivity curves of Macroporous Bead Cellulose MT types Kav vs. Molecular Weight of globular proteins in semilogarithmic scale
Elution behavior of globular protein on Macroporous Bead Cellulose MT, dependence of distribution coefficient Kav on Molecular Weight
eluent: buffer 0.2 M phosphate pH 7.0 *) buffer 0.013 M phosphate pH 7.0
Selectivity curves of Macroporous Bead Cellulose MT types Kav vs. Molecular Weight of Dextran in semilogarithmic scale
Elution behavior of Dextran on Macroporous Bead Cellulose MT, dependence of distribution coefficient Kav on Molecular Weight
It is necessary to mention that the pore volume accessible to a given molecule is determined by its size (weight) and the pore size distribution of packing and can be defined by the following formula:
where Kav is the distribution coefficient (the factor of pore volume available for a given molecule), Ve is elution (retention) volume, Vo is void (interstitial) volume and Vt is total column volume.
Selectivity parameters of Macroporous Bead Cellulose MT
Other Technical Data
Temperature Resistance (2) oC : 120
Stability within pH Range : 1 - 14 NaOH or HCl (c = 1 mol / l)
Stability in Salt Solutions : with ionic strength up to 10 mol/l
Swelling / Shrinkage (vol %) :
Chemical Resistance : aqueous
solutions, buffer, organics, detergents
(2) In wet state, pH 7.0 for 1 hour.
Handling with Macroporous Bead Cellulose and Sorbents IONTOSORB
Bead Cellulose and its derivatives must not be dried, because drying results in loss of porosity. Therefore they are supplied fully swollen in water, sucked off. They can be stabilized by sodium azide (0.02 %) or ethanol (20 %) according to customer┤s demand.